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Proteins were fractionated electrophoretically on NuPAGETM 412% Bis-Tris Polyacrylamide Gel (Invitrogen) and then transferred to a polyvinylidene fluoride (PVDF) membrane using the iBlot Dry Blotting System (Invitrogen)

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Many patients benefit from a gradual dose escalation protocol, often called microdosing, to minimize side effects and allow their bodies to adapt to the medication's appetite-suppressing effects

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