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Diabetes 64(3):819827 Coskun T, Sloop KW, Loghin C, Alsina-Fernandez J, Urva S, Bokvist KB et al (2018) LY3298176, a novel dual GIP and GLP-1 receptor agonist for the treatment of type 2 diabetes mellitus: from discovery to clinical proof of concept
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247 Mechanistically, it is unclear how GLP-1-based therapies exert beneficial effects on the cardiovascular system, but studies have suggested that this is achieved by direct (cardiovascular GLP-1R activation) and indirect (promotion of the incretin effect) mechanisms
HEK293T cells (obtained from and certified by the Cell Bank at the Chinese Academy of Sciences and confirmed as negative for mycoplasma contamination) were cultured in DMEM supplemented with 10% (v/v) foetal bovine serum, 50 IU mL 1 penicillin and 50 g mL 1 streptomycin
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