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One other important question regarding detecting the sKlotho level is how to distinguish the sources of sKlotho due to the presence of two isoforms in the circulation

Pozsgai E, Schally AV, Zarandi M, Varga JL, Vidaurre I, Bellyei S

FoB were activated with 5 g/mL anti-IgM (Jackson Immunoresearch 715-006-020), 50 ng/mL CD40 ligand (Bio-techne 8230-CL-050) and 10 ng/mL IL-4 (Miltenyi Biotec 130-097-757)