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The measurements were done in myocytes superfused with Tyrode's solution (1.2 mM Ca 2+ ) at 3536C and paced with field stimulation at 1 Hz as previously described (26, 30, 31)

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Mechanisms: It enhances physiologic GH pulsatility, particularly during sleep cycles, supporting recovery, protein synthesis, and tissue repair without overstimulation or endocrine disruption

The rest were continued experiment for 8 weeks of treatment and followed by echocardiography examination

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