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To develop liver fibrosis, rats in Group 2, the TAA group, received IP injections of TAA (100 mg/kg b.wt), twice weekly for 6 weeks ( 2.4 Preparation of blood and liver tissues Twenty-four hours after the last injection, blood was obtained from the tail vein under anesthesia with intraperitoneal injections of ketamine (50 mg/kg) + xylazine (25 mg/kg) dissolved in saline