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Description

Follow a cycle of ~8 weeks on, then 24 weeks off.

Increased water and salt retention

Fifty microliters of standard solutions were mixed with assay cocktail (12.5 l of 4 mM NADPH in 5% NaHCO 3, 25 l of GSH reductase solution (Roche, No.105678, 5 mg/ml), 12.5 l of 10 mM 5,5-dithiobis-2-nitrobenzoic acid in 0.1 M phosphate buffer, 75 l MES buffer, 25 l distilled water) on 96-well plate