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Collect these fractions this is where youre expecting your protein of interest and any interacting partner proteins to be
(4)Fmoc-Ala-Gly-Leu-Val- (4) Preparation of Fmoc-Ala-Gly-Leu-Val-resin: (3)Fmoc-Gly-Leu-Val-1050530105010603DMF3DMF2DMFFmoc-Ala-OHDIEATBTU/HOBtHBTU/HOBtBOP/HOBt105010603DMF3DMF2Fmoc-Ala-Gly-Leu-Val- In the Fmoc-Gly-Leu-Val-resin of step (3), add decapping reagent 1050 and react for 530 minutes, use vacuum pump to dry, add decapping reagent 1050 and react for 1060 minutes, pump Dry, wash 3 times with isopropanol, 3 times with DMF, 2 times with redistilled DMF, add a mixture of Fmoc-Ala-OH, DIEA, TBTU/HOBt or HBTU/HOBt or BOP/HOBt dissolved in DMF , reacted at 10-50C for 10-60 minutes, pumped dry with a vacuum pump, washed 3 times with isopropanol, washed 3 times with DMF, washed 2 times with distilled DMF, and dried to obtain Fmoc-Ala-Gly-Leu- Val-resin
