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All mice were housed under a 12 h light/dark cycle, with the temperature consistently maintained between 18 C and 23 C and the humidity kept at ~50%

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Curves presented the least squares (LS) means of 10th, 25th, 50th (median), 75th, and 90th percentile of IGF-I and IGFBP-3 calculated by the generalized additive model (GAM) and quantile regression