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The primary antibodies used were against ILF3 (ab92355, Abcam, UK), SLC7A11 (ab307601, Abcam, UK), GPX4 (ab252833, Abcam, UK), GAPDH (ABclonal, A19056), PCNA (ab92552, Abcam, UK), HDAC1 (A19571, ABclonal, China), HDAC2 (A19626, ABclonal, China), HDAC6 (A3572, ABclonal, China), DEPTOR (A9447, ABclonal, China), mTOR (2972S, Cell Signaling Technology, USA), p-mTOR (5536S, Cell Signaling Technology, USA), H3K14ac (A7254, ABclonal, China), PD-L1 (66248-1-Ig, Proteintech, China), CD8 (66868-1-Ig, Proteintech, China), pan acetyl lysine (PTM-105, PTM Bio, China), and 4-hydroxynonenal (4-HNE) (bs-6313R, Bioss, China)
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The results demonstrated that, compared with the normoxia group, hypoxia significantly enhanced the expression of key genes in the HIF-1 signaling pathway ( ENO2 , HK2 , SLC2A1 , and PFKP ), and inhibited the expression of genes associated with the Hippo signaling pathway ( WTIP , CCN2 , NF2 , and AXIN2 ) (Figure 6e)
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