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Daily whole-well imaging using an Incucyte live-cell imaging system (Essen Bioscience) was performed to analyze wound closure and the area of the wound was measured using Fiji ImageJ software

Importantly, either genetic silencing of NCOA4 or treatment with the iron chelator deferoxamine (DFO) significantly attenuated these pathological changes, resulting in over 60% improvement in neuronal survival (Plascencia-Villa and Perry, 2021)

Another study, on MK-677, published in the Journal of Clinical Endocrinology & Metabolism , showed it boosted growth-hormone levels in real live older folks to the normal range found in young adults