However, long-term studies specifically tracking extended use are lacking
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Western blot analysis Cells CAOV3 cells (2.5 10) were lysed in 200 L of RIPA buffer (Sigma, R02778) supplemented with protease inhibitor cocktail (Roche, 04693159001) and mixed at 1500 rpm for 30 min at 4 C using a MixMate (Eppendorf)
24 A more recent double-blind, randomized, controlled trial that investigated the microvascular effects of linagliptin in nondiabetic patients with arterial hypertension resulted in a significant improvement in retinal capillary perfusion and retinal arterial blood flow