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The homogenates were filtered through 100 m cell strainers and filters were washed twice with FACS buffer (1 mM EDTA, 25 mM HEPES, 1% FBS in PBS) and centrifuged at 500 g for 10 min at 4 C

Normally, Cyp2e1 converts alcohol to acetaldehyde, and then another enzyme (aldehyde dehydrogenase) converts acetaldehyde to harmless acetate

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