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Rapid weight gain over days to weeks , especially with swelling or breathlessness, warrants prompt medical review

Injection into areas with less subcutaneous fat or closer proximity to nerve endings may also increase pain perception

The samples were centrifuged, and the supernatant was subjected to a GSH assay based on a kinetic enzymatic recycling method that detects the oxidation of GSH by 5,5-dithiobis-2-nitrobenzoic acid (DTNB) and glutathione reductase to measure the GSH content in cells 74

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