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The concentration of anti-inflammatory and inflammatory cytokines was detected using LEGENDplex TM Rat Inflammatory Panel (Biolegend) or LEGENDplex TM Customer Panel (Biolegend) following the manufacturer's protocol and acquired via BD FACSAria III cell sorter (BD Biosciences, USA)

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IGF-1 LR3 activates PI3K/Akt - the same insulin signalling cascade

Moreover, previous research in both mice and rats suggests that the effects of caffeine are more pronounced in preventing memory deterioration rather than enhancing memory in rodents not exposed to potentially harmful stimuli (Cunha et al., 2016)