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The complex was solubilized from the membrane by 0.5% (w/v) lauryl maltose neopentyl glycol (LMNG, Anatrace) supplemented with 0.03% (w/v) cholesteryl hemisuccinate (CHS, Anatrace) for 1 h at 4 C

In addition, exacerbation of the reduction in ATP and observed phosphocreatine levels, is accompanied by an increase in ADP and orthophosphate levels, in both cardiac tissue and other organs (30)

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