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GHK-Cu naturally declines as we age

Stohl W, Hilbert DM

For anti-C1q or anti-NeuN antibody staining, a DAKO EnVision + System (Agilent Technologies, Santa Clara, CA, USA) was used as the second antibody and the signal was detected using tyramide-enhanced fluorescein isothiocyanate (FITC), as for the TSA method

However, the median follicular Hcy concentration of cases was just 30% of that recorded in their controls, which warrants better sized studies to clarify this point