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Comparing the sgRNA distribution in the non-treated cells with the library plasmid stock showed that several sgRNAs targeting essential control genes and essential membrane transporters led to a growth reduction (Fig S2B), confirming a high KO efficiency in this cell line and experimental setup

Moreover, GPX4 levels were elevated on treatment with SCLA2 and derived alkyne probes SCLA2a and SCLA2b (Extended Data Fig

This work was supported by grants from the Deutsche Forschungsgemeinschaft to BG (GR 936/171 and 17-2) and by the Collaborative Research Center SFB1078 (Humboldt Universitt zu Berlin, TP A5 to RH)