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After washed twice with cold PBS, the cells were suspended with 200 l of PBS, and supplemented with 10 l of RNase A stock (#10405ES03, Yeasen) solution and incubated 60 min at room temperature

ProtocolGuinea Pig Female albino guinea pigs rats (n=6 per group) of approximately 6-8 weeks of age served as subjects for the experiments

This may happen because glutathione interacts with detoxification pathways in the liver, which temporarily trigger mild digestive upset

However, while strengthening research on biomarkers is essential, these discoveries can only translate into meaningful clinical benefit if accompanied by improvements in healthcare coverage that facilitate timely diagnosis, treatment, and follow-up for the broader population

The GST response in group 5 was in parallel to the response of group 4 with slight elevation until day 15