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Because zebrafish eggs are externally fertilized and their embryos are optically clear, cellular behaviors can be imaged in live animals

KLOW = KPV + the GLOW three

This might possibly be due to the engineered control circuits responding to the target product intermediate, limiting any improvement in production beyond initial control levels

However, due to the disruption of organelle/cell integrity followed by a mixing of compartments and oxidation of GSH to GSSG, such estimates lack sub-cellular relevance [28], and dynamic measurements are impossible

At least 70 enzymes have been identified as requiring CoA or ACP derivatives for their function including the same critical thiamine-dependent enzymes described above, the pyruvate dehydrogenase complex (PDHc), 2-oxoglutarate (-ketoglutarate) dehydrogenase complex (OGDH), and branched-chain -ketoacid dehydrogenase complex (BCKD)