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Solutions: Use a higher concentration with smaller reconstitution volume, which does not actually solve the stability issue since the same amount of time passes Start at a higher dose point if your protocol allows (only if you have already titrated up with a smaller vial) Consider aliquoting into smaller sterile vials and freezing portions for later use (reduces contamination risk to the main vial) Share the reconstitution timing with your dosing schedule, only reconstituting when you are at a dose where you will use the vial within 28-60 days For researchers at 10 mg or 15 mg weekly, the 60 mg vial is perfect

Thus, we define our experimentally-measured crowding free energy for a given sensor as a mean energy penalty U , which can be predicted by weighting the FITC 1-D probability density P FITC by the crowding penalty U and integrating across all space: To describe P FITC , we invoke the continuous Gaussian chain model of a surface-tethered polymer of mean height h in an ideal solvent, calculating the chain-end distribution (see Supporting Information for calculations) 34