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BioLegend) in U-bottom 96-well plates

Archived from the original on 28 February 2008

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Improvements were also seen in blood pressure, glycemic parameters, and physical function

For cell proliferation assays with fold change in cell number, cells were cultured in replicates of 3 in 96-well plates at 1000 (suspension) or 150 (adherent) cells per well in 200 uL RPMI or DMEM base media under the conditions described in each experiment, and a separate group of 3 wells was also plated for each cell line with no treatment for an initial time point