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Cell culture The human ovarian granulosa cell line KGN (Procell, Wuhan, China) was cultured in Dulbecco's modified Eagle's medium (DMEM)-F12 medium (GIBCO-BRL, Invitrogen) containing 10% fetal bovine serum (Invitrogen) and 1% penicillinstreptomycin (Invitrogen) at 37C under 5% CO
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The solution: Remember the formula: Volume (ml) x Concentration (mg/ml) = Dose (mg)
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May Support Liver-Related Nutrient Pathways Choline is involved in lipid transport and metabolism, and the body uses choline to help produce phosphatidylcholine, a major phospholipid needed for cell membranes
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