Quantitative RT-PCR RNA was isolated using a NucleoSpin kit (Machery-Nagel, 740955.50) and converted in cDNA using an iScript cDNA Synthesis Kit (Biorad)
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In the foregoing synthesis method of cagriltide, the specific step of cyclization in step four includes: dissolving the linear peptide crude product in acetonitrile water according to the dose that 1g of the linear peptide crude product is dissolved in 100mL of 50% acetonitrile water solution, adding purified water to dilute the linear peptide crude product to 1g/L after the linear peptide is dissolved
The kit can also be used to measure only GSSG by following an alternative protocol
Following the study, the following was reported: (1) The peptide appeared to promote the outgrowth of tendon fibroblasts and tissue healing
