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In vitro transcription with 3-Cy3 labelling Cold in vitro transcription was performed in a volume of 50 l containing 1 g DNA template (annealed oligonucleotides), 1 mM NTPs solution, 100 U RiboLock RNase Inhibitor (Thermo Fisher Scientific), 1x Transcription Buffer and 500 U T7 RNA Polymerase (NEB)