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First and foremost, the extracellular molecules that can be analyzed with this approach is limited to only those that have natural receptors or achieve cell entry via infection

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IGF-1 induces hypoxia-inducible factor 1-mediated GLUT3 expression through PI3K/Akt/mTOR dependent pathways in PC12 cells

Whats next in the GLP-1 pipeline

Frozen slices 12 mm thick were blocked with PBS + 5% NGS, labeled with a Rat Monoclonal Antibody for CD54eBioKAT-1from eBioscience, Invitrogen diluted 1:200 in blocking buffer, then with an Alexa 488 Goat anti-Rat IgG secondary antibody diluted 1:500 in blocking buffer and counterstained with DAPI, and mounted in Vectashield mounting media for fluorescence