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The residues were mixed with 20 L of 20 mM HCl, 60 L of the sodium borate buffer provided with the AccQ Tag Ultra Derivatization Kit, and 20 L of 0.3% (w/v) AccQ acetonitrile solution and heated at 50 C for 10 min to obtain their AccQ derivatives

The following are the detailed synthesis steps and key points: Raw materials: involving various amino acids, such as Nalpha-FMOC-Nepsilon-Boc-L-lysine,FMOC-D-phenylalanine,FMOC-D-2-naphthylalanine,FMOC-Npi-trityl-L-histidine Wait, and 2- (tert butoxycarbonylamino) isobutyric acid