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You can calculate the concentration of GLP1 in the samples by comparing the OD of the samples to the standard curve

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Diabetes Obes Metab 26(Suppl 4):1627 Xiang J, Qin L, Zhong J, Xia N, Liang Y (2023) GLP-1RA liraglutide and semaglutide improves Obesity-Induced muscle atrophy via SIRT1 pathway

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Clinical trials with hundreds or thousands of participants provide statistical power to identify patterns

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