Generally, GSH binding shows relatively strong affinity to the G-site (near the active cysteine residue) and weak affinity to the H-site in the case of WT proteins
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[DOI] [PMC free article] [PubMed] [Google Scholar] 14.Tsujino S., Masuda R., Shimizu Y., Azuma Y., Kanada Y., Fujiwara T
This percentage will be even less in more acidic compartments such as the vacuole or apoplast, although GSH concentrations are relatively low at these locations