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The PCR amplification of transposon-genome junctions was performed using the cycling parameters described in the kit for 11 cycles with Q5 Ultra II FS Master Mix (NEB) using primers YL006 (AGCGGCAATTTCACACAGGA) and JBD-SS-272/oAD493 (ACACTGGCAGAGCATTACGCCCT) instead of the kit-supplied primers

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1 Characterization of SK-N-SH cells and genome-wide CRISPR library fitness parameters

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