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however, hCG was not detected owing to a poor ionization in the ESI source and high heterogeneity of hCG isoforms, consistent with previously published studies (Camperi et al., 2018)

To evaluate the IC 50 , the culture medium was removed, and the cells were cultured for additional 24 h with: i) complete DMEM

For example, they may be scared to eat because they think theyll gain weight, or they wait until theyre very hungry before they eat