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The purity of the lyophilized peptide is the true starting point

Mutations identified in patients with defective lipoic acid metabolism affect genes involved in the synthesis of iron-sulfur clusters and genes coding for lipoic acid synthetase ( LIAS ), lipoyl transferase 1 ( LIPT1 ), and dihydrolipoamide dehydrogenase (E3 component of -ketoacid dehydrogenase complexes

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Furthermore, preliminary tests allowed the determination of the maximum control current and the appropriate time extent of the irradiation in order to keep the sample temperature in the physiological range