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reinhardtii variant ( Cr GPDH) decreased the total cellular NADH/NAD + ratio while its expression did not affect the total cellular NADPH/NADP + ratio ( Figure 1e-f , Supplementary Figure S3b-g )

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Western Blotting Proteins were extracted from chondrocyte (in vitro) and cartilage (in vivo) with cell lysis buffer (Pro-Prep, Intron Biotechnology, Korea) and protein concentration was measured using a BCA kit (Thermo Scientific, USA)

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Each of the existing structure-activity relationship (SAR) models proposes structural alerts, i.e., moieties associated with sensitizing activity