California is an enormous state, with large cities, tiny towns and all the rest between
Choose oral, not IV, if kidney safety is a priority
Discarding the upper plasma, wash with three times volume of PBS for 3 times (re-suspend blood cell with PBS, centrifuge at 600 g for 10 minutes), add equal volume of Reagent I
SSZ and RAM provided senior author input in the design of the study and review of the manuscript, and UA oversaw the study development, manuscript writing, provided senior author review of the manuscript and is the guarantor of this work
(Make sure that you evaluate your levels with the optimal range and not just the normal range in mind please see the link above for further details.) One of the most frustrating parts of diagnosing and managing Thyroid resistance with Leptin resistance is that your thyroid lab tests may always show up as normal